Qualité microbiologique des tranches de mangues (Mangifera indica L.) vendues à Dakar (Sénégal)

La mangue, principal fruit du Sénégal, est transformée en tranches et vendue en sachets dans les rues de Dakar. Ce produit est sensible aux altérations microbiennes faute de technologies de conservation adaptées au contexte local. Ce travail propose une évaluation de la qualité  microbiologique du produit et de son impact sur la santé des  consommateurs. Pour cette étude, 3 répétitions d’échantillons par vendeur ont été prélevées chez quatorze vendeurs de cinq quartiers (Médina, Fann, Habitats Loyers Modernes ou HLM, Grand Yoff et Guédiawaye). Ensuite, des analyses ont été menées pour la recherche de la flore mésophile aérobie totale, de coliformes, d’entérobactéries et d’Escherichia coli présomptifs. Les résultats montrent une forte pollution des échantillons en flore totale sauf pour l’échantillon V3 (vendeur 3) des HLM et une charge élevée en coliformes et en Escherichia coli (> 103 UFC/g). D’autres entérobactéries comme Enterobacter agglomerans, Enterobacter amnigenus, Serrati  rubidaea et Klebsiella pneumoniae sont retrouvées respectivement dans les échantillons V2 de Médina, V1 de Fann, V1 et V2 Guédiawaye. Ces bactéries ne présentent pas de risques sur la santé publique mais rendent le produit impropre à la consommation. Cette étude nécessite une expansion aux autres quartiers pour mieux veiller sur la santé des consommateurs.Mots clés: Evaluation, qualité, microbiologie, mangues, santé des consommateurs, Dakar

Use of edible and biodegradable coatings in fresh cut pineapple.

The use of edible coatings in vegetable products has been increasing because it offers several advantages as being biodegradable and extends the shelf-life of fruits and vegetables. The objective of this work was to evaluate the effect of two biodegradable edible coatings, sodiumalginate and Chitosan, on the quality and shelf life of minimally processed pineapple storage at 10°C. Five L of each coating solutions were prepared as following - 2% alginate solution: 100 g of Algenic acid sodium salt in 5 L of distilled water, heated at 70°C under constant stirring - 0,75% Chitosan solution: 150 g of monohydrated citric acid dispersed in 5 L of distilled water and addition 37.5g of Chitosan (Crab shells Chitosan, minimum 85% deacetylated, SIGMA), heated to 60°C to dissolve the Chitosan. The coating solutions were then stored at 5°C before the dipping. The Chitosan coating had an important benefic effect on the microbiological contamination and on the maintenance of the total phenolic content. The high concentration of citric acid in the coating solution contributed to the antimicrobial effect but changed the sensory taste of fresh pineapple. The alginate coating also decreased the contamination of yeasts and molds but its effect was lower than the Chitosan one. Visual aspect of its coating solution can be not well accepted by the consumers. The concentration of alginate must be decreased to avoid the formation of a too thick gel. The Chitosan treatment resulted in the best product at the end of experiment and the most slight decreasing of phenolic compounds during all the experiments

Mutation in human CLPX elevates levels of δ-aminolevulinate synthase and protoporphyrin IX to promote erythropoietic protoporphyria

Loss-of-function mutations in genes for heme biosynthetic enzymes can give rise to congenital porphyrias, eight forms of which have been described. The genetic penetrance of the porphyrias is clinically variable, underscoring the role of additional causative, contributing, and modifier genes. We previously discovered that the mitochondrial AAA+ unfoldase ClpX promotes heme biosynthesis by activation of δ-aminolevulinate synthase (ALAS), which catalyzes the first step of heme synthesis. CLPX has also been reported to mediate heme-induced turnover of ALAS. Here we report a dominant mutation in the ATPase active site of human CLPX, p.Gly298Asp, that results in pathological accumulation of the heme biosynthesis intermediate protoporphyrin IX (PPIX). Amassing of PPIX in erythroid cells promotes erythropoietic protoporphyria (EPP) in the affected family. The mutation in CLPX inactivates its ATPase activity, resulting in coassembly of mutant and WT protomers to form an enzyme with reduced activity. The presence of low-activity CLPX increases the posttranslational stability of ALAS, causing increased ALAS protein and ALA levels, leading to abnormal accumulation of PPIX. Our results thus identify an additional molecular mechanism underlying the development of EPP and further our understanding of the multiple mechanisms by which CLPX controls heme metabolism. Keywords: heme biosynthesis; porphyria; ALAS; protein unfoldases; AAA+ ATPaseNational Institutes of Health (U.S.) (Grant F32 DK095726)National Institutes of Health (U.S.) (Grant R01 GM049224

Melting of hybrid organic-inorganic perovskites.

Several organic-inorganic hybrid materials from the metal-organic framework (MOF) family have been shown to form stable liquids at high temperatures. Quenching then results in the formation of melt-quenched MOF glasses that retain the three-dimensional coordination bonding of the crystalline phase. These hybrid glasses have intriguing properties and could find practical applications, yet the melt-quench phenomenon has so far remained limited to a few MOF structures. Here we turn to hybrid organic-inorganic perovskites-which occupy a prominent position within materials chemistry owing to their functional properties such as ion transport, photoconductivity, ferroelectricity and multiferroicity-and show that a series of dicyanamide-based hybrid organic-inorganic perovskites undergo melting. Our combined experimental-computational approach demonstrates that, on quenching, they form glasses that largely retain their solid-state inorganic-organic connectivity. The resulting materials show very low thermal conductivities (~0.2 W m-1 K-1), moderate electrical conductivities (10-3-10-5 S m-1) and polymer-like thermomechanical properties

ESKIMO1 Disruption in Arabidopsis Alters Vascular Tissue and Impairs Water Transport

Water economy in agricultural practices is an issue that is being addressed through studies aimed at understanding both plant water-use efficiency (WUE), i.e. biomass produced per water consumed, and responses to water shortage. In the model species Arabidopsis thaliana, the ESKIMO1 (ESK1) gene has been described as involved in freezing, cold and salt tolerance as well as in water economy: esk1 mutants have very low evapo-transpiration rates and high water-use efficiency. In order to establish ESK1 function, detailed characterization of esk1 mutants has been carried out. The stress hormone ABA (abscisic acid) was present at high levels in esk1 compared to wild type, nevertheless, the weak water loss of esk1 was independent of stomata closure through ABA biosynthesis, as combining mutant in this pathway with esk1 led to additive phenotypes. Measurement of root hydraulic conductivity suggests that the esk1 vegetative apparatus suffers water deficit due to a defect in water transport. ESK1 promoter-driven reporter gene expression was observed in xylem and fibers, the vascular tissue responsible for the transport of water and mineral nutrients from the soil to the shoots, via the roots. Moreover, in cross sections of hypocotyls, roots and stems, esk1 xylem vessels were collapsed. Finally, using Fourier-Transform Infrared (FTIR) spectroscopy, severe chemical modifications of xylem cell wall composition were highlighted in the esk1 mutants. Taken together our findings show that ESK1 is necessary for the production of functional xylem vessels, through its implication in the laying down of secondary cell wall components

Herkules – Held zwischen Tugend und Hybris. Ein europäischer Erinnerungsort der Frühen Neuzeit?

This essay traces some of the contexts and media in which "Heracles-Hercules" - as a hero between virtue and hubris - was visible in European societies from the end of the middle ages onwards. It discusses whether this example of the reception, appropriation and transformation of classical myths in the early modern period can be understood as a European "lieu de mémoire", and to what extent the concept of "lieux de mémoire" (or sites of memory) can foster our understanding of a particular object of study (here the Heracles/Hercules myth). Against this backdrop, we conclude with some general questions about the more ambitious project* of a comprehensive register of European "lieux de mémoire". * * Published as: Boer, Pim den / Duchhardt, Heinz / Kreis, Georg / Schmale, Wolfgang (eds.): Europäische Erinnerungsorte, 3 vols., Munich 2011–201